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Comparison of iodine-123 low-density lipoprotein (LDL) and indium-111 LDL binding to mononuclear cells of healthy normolipaemic controls and patients with heterozygous familial hypercholesterolaemia

Identifieur interne : 000206 ( Main/Exploration ); précédent : 000205; suivant : 000207

Comparison of iodine-123 low-density lipoprotein (LDL) and indium-111 LDL binding to mononuclear cells of healthy normolipaemic controls and patients with heterozygous familial hypercholesterolaemia

Auteurs : RBID : ISTEX:259_1994_Article_BF00285585.pdf

English descriptors

KwdEn :
- Familial hypercholesterolaemia, Indium-111 labelling, Iodine-123 labelling, Low-density lipoprotein, Peripheral mononuclear cells.

Abstract

The binding of radiolabelled lipoproteins, iodine-123-labelled low-density. lipoprotein (LDL) and indium-111-labelled LDL, to peripheral blood mononuclear cells (MNCs) was compared in normolipaemic subjects and in patients with heterozygous familial hypercholesterolaemia (FH). 123I-LDL and 111In-LDL binding to MNCs exhibited high-affinity, highly specific, time- and temperature-dependent binding reaching saturation at concentrations above 50 nM. The number of LDL binding sites (Bmax) was significantly (P<0.01) lower in FH patients (P<0.001; 123I-LDL: Bmax 279±44 ng protein/108MNCs; 111In-LDL: Bmax 309±43 ng protein/108MNCs) as compared with controls (123I-LDL: Bmax 2874±246 ng protein/108 MNCs; 111In-LDL: Bmax 3145±339 ng protein/108 MNCs). The corresponding dissociation constants (Kd) were 16±8 nM for 123I-LDL and 12±6 nM for 123In-LDL in healthy volunteers (123In-LDL vs 111In-LDL, P<0.05). In FH patients, the Kd values were 20±8 nM for 123I-LDL and 16±6 nM for 123In-LDL (P<0.05 vs controls for both 123I-LDL and 111In-LDL). 111In-LDL binding to MNCs was inhibited (IC50) by 30±8 nM in healthy controls and 38±12 nM in FH patients (P<0.05). 123In-LDL binding to MNCs was inhibited (IC50) by 34±8 nM in healthy controls and 46±10 nM in FH patients (P<0.05). Taken together, these results suggest a reduced number of LDL receptors expressed on MNCs from FH patients. We conclude that 111In-LDL and 123I-LDL are equally well suited as a probe of receptor-mediated binding and uptake of LDL.

DOI: 10.1007/BF00285585

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<author><name>M. Banyai</name>
<affiliation wicri:level="1"><mods:affiliation>Department of Nuclear Medicine, University of Vienna, Vienna, Austria</mods:affiliation>
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<author><name>G. Lupattelli</name>
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<author><name>S. R. Li</name>
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<author><name>Q. Yang</name>
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<front><div type="abstract" xml:lang="eng">The binding of radiolabelled lipoproteins, iodine-123-labelled low-density. lipoprotein (LDL) and indium-111-labelled LDL, to peripheral blood mononuclear cells (MNCs) was compared in normolipaemic subjects and in patients with heterozygous familial hypercholesterolaemia (FH). 123I-LDL and 111In-LDL binding to MNCs exhibited high-affinity, highly specific, time- and temperature-dependent binding reaching saturation at concentrations above 50 nM. The number of LDL binding sites (Bmax) was significantly (P<0.01) lower in FH patients (P<0.001; 123I-LDL: Bmax 279±44 ng protein/108MNCs; 111In-LDL: Bmax 309±43 ng protein/108MNCs) as compared with controls (123I-LDL: Bmax 2874±246 ng protein/108 MNCs; 111In-LDL: Bmax 3145±339 ng protein/108 MNCs). The corresponding dissociation constants (Kd) were 16±8 nM for 123I-LDL and 12±6 nM for 123In-LDL in healthy volunteers (123In-LDL vs 111In-LDL, P<0.05). In FH patients, the Kd values were 20±8 nM for 123I-LDL and 16±6 nM for 123In-LDL (P<0.05 vs controls for both 123I-LDL and 111In-LDL). 111In-LDL binding to MNCs was inhibited (IC50) by 30±8 nM in healthy controls and 38±12 nM in FH patients (P<0.05). 123In-LDL binding to MNCs was inhibited (IC50) by 34±8 nM in healthy controls and 46±10 nM in FH patients (P<0.05). Taken together, these results suggest a reduced number of LDL receptors expressed on MNCs from FH patients. We conclude that 111In-LDL and 123I-LDL are equally well suited as a probe of receptor-mediated binding and uptake of LDL.</div>
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<abstract lang="eng">The binding of radiolabelled lipoproteins, iodine-123-labelled low-density. lipoprotein (LDL) and indium-111-labelled LDL, to peripheral blood mononuclear cells (MNCs) was compared in normolipaemic subjects and in patients with heterozygous familial hypercholesterolaemia (FH). 123I-LDL and 111In-LDL binding to MNCs exhibited high-affinity, highly specific, time- and temperature-dependent binding reaching saturation at concentrations above 50 nM. The number of LDL binding sites (Bmax) was significantly (P<0.01) lower in FH patients (P<0.001; 123I-LDL: Bmax 279±44 ng protein/108MNCs; 111In-LDL: Bmax 309±43 ng protein/108MNCs) as compared with controls (123I-LDL: Bmax 2874±246 ng protein/108 MNCs; 111In-LDL: Bmax 3145±339 ng protein/108 MNCs). The corresponding dissociation constants (Kd) were 16±8 nM for 123I-LDL and 12±6 nM for 123In-LDL in healthy volunteers (123In-LDL vs 111In-LDL, P<0.05). In FH patients, the Kd values were 20±8 nM for 123I-LDL and 16±6 nM for 123In-LDL (P<0.05 vs controls for both 123I-LDL and 111In-LDL). 111In-LDL binding to MNCs was inhibited (IC50) by 30±8 nM in healthy controls and 38±12 nM in FH patients (P<0.05). 123In-LDL binding to MNCs was inhibited (IC50) by 34±8 nM in healthy controls and 46±10 nM in FH patients (P<0.05). Taken together, these results suggest a reduced number of LDL receptors expressed on MNCs from FH patients. We conclude that 111In-LDL and 123I-LDL are equally well suited as a probe of receptor-mediated binding and uptake of LDL.</abstract>
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Comparison of iodine-123 low-density lipoprotein (LDL) and indium-111 LDL binding to mononuclear cells of healthy normolipaemic controls and patients with heterozygous familial hypercholesterolaemia

Comparison of iodine-123 low-density lipoprotein (LDL) and indium-111 LDL binding to mononuclear cells of healthy normolipaemic controls and patients with heterozygous familial hypercholesterolaemia

English descriptors

Abstract

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